Chemical analyses protocols
Analyses and determinations:
- Hydroxycinnamic acids: soluble, ester-conjugates, glicosilates, and eter- and esterified to cell wall.
- Hydroxamic acids: DIMBOA, HMBOA, MBOA
- Cell wall content (gravimetry)
- Acid detergent lignin (ADL) and Klason lignin (KL)
- Acid detergent fiber (ADF)
- Neutral Detergent Fiber (NDF)
- Lignin subunits (S, G y H) by thioacidolysis or nitrobenzene oxidation
- Total soluble phenols (Folin-Ciocalteu)
- Total and individual flavonoids
- Total anthocyanes
- Total and individual soluble sugars
- Structural polysaccharide sugars: neutral, total (Phenol-Sulphuric), uronic acids (Blumenkrantz), cellulose (Updegraff-Antrona), sugar % composition and fractioning
- Protein content (Bradford)
- Photosynthetic pigments (spectrophotometry)
- Ergosterol
- Fumonisin (B1 y B2)
Please, contact us if you are interested in some of these analyses.
Plant defense against biotic stresses
Associated Unit BEV1, University of Vigo - Misión Biológica de Galicia (CSIC)
Associated Unit BEV1, University of Vigo - Misión Biológica de Galicia (CSIC)
