Plant defense against biotic stresses
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Chemical analyses protocols


Analyses and determinations:
  • Hydroxycinnamic acids: soluble, ester-conjugates, glicosilates, and eter- and esterified to cell wall.
  • Hydroxamic acids: DIMBOA, HMBOA, MBOA
  • Cell wall content (gravimetry)
  • Acid detergent lignin (ADL) and Klason lignin (KL)
  • Acid detergent fiber (ADF)
  • Neutral Detergent Fiber (NDF)
  • Lignin subunits (S, G y H) by thioacidolysis or nitrobenzene oxidation
  • Total soluble phenols (Folin-Ciocalteu) 
  • Total and individual flavonoids
  • Total anthocyanes
  • Total and individual soluble sugars
  • Structural polysaccharide sugars: neutral, total (Phenol-Sulphuric), uronic acids (Blumenkrantz), cellulose (Updegraff-Antrona), sugar % composition and fractioning
  • Protein content (Bradford)
  • Photosynthetic pigments (spectrophotometry)
  • Ergosterol
  • Fumonisin (B1 y B2)

Please, contact us if you are interested in some of these analyses.

Plant defense against biotic stresses
Associated Unit BEV1, University of Vigo - Misión Biológica de Galicia (CSIC)
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